UniversityFacultyInstitute
Prof. Dr. Reinhard Krämer

tel.: 0221 / 470 64 61

fax.: 0221 / 470 50 91

email.: r.kraemer"at"uni-koeln.de

Institut für Biochemie,
Zülpicher Str. 47 50674 Köln

Prof. Dr. Reinhard Krämer

We mainly focus on two different aspects of molecular biology, biochemistry and physiology of microorganisms:

Signal transduction pathways related to global regulatory networks of nutrient limitation and stress response.The signal transduction cascade(s) and global regulatory networks of osmoregulation and temperature stress in Corynebacterium glutamicum and in Synecchocystis sp. 6803 are studied on the level of functional genomics, proteomics, protein-protein interaction, biochemical mechanisms, as well as physiological significance. As a particular interest, the mechanisms of stimulus sensing in relation to the cell's response to osmotic stress is analyzed.

Structure, function, regulation and biotechnological significance of membrane transport systems. We are interested in structure, function and regulation (on the level of both protein activity and gene expression) of osmoregulated transporters for osmoprotectant substances in the two organisms studied, Corynebacterium glutamicum and in Synecchocystis sp. 6803. In cooperation with industrial partners, we are also studying carrier systems responsible for the excretion of substances with biotechnological relevance, e.g. amino acids and vitamins, both by C. glutamicum and Escherichia coli.

In order to solve these scientific questions the proteins and genes of interest are studied by techniques of molecular genetics (transposon mutagenesis, promoter analysis, RNA hybridization experiments, DNA microarrays etc.), by biochemical approaches (functional characterization of transport systems, isolation, purification and reconstitution of (membrane) proteins, analysis of protein-protein interactions, proteomic and immunological techniques), various spectroscopic methods, and techniques of microbiology/ biotechnology (batch, fed-batch, and continuous fermentation).