Publications in 1997

Schroers, A., Kramer, R., and Wohlrab, H. (1997)

The reversible antiport-uniport conversion of the phosphate carrier from yeast mitochondria depends on the presence of a single cysteine
J.Biol.Chem. 272, 10558-10564

Soboll, S., Conrad, A., Eistert, A., Herick, K., and Kramer, R. (1997)

Uptake of creatine phosphate into heart mitochondria: a leak in the creatine shuttle
Biochim.Biophys.Acta 1320, 27-33

Herick, K., Kramer, R., and Luehring, H. (1997)

Patch clamp investigation into the phosphate carrier from Saccharomyces cerevisiae mitochondria
Biochim.Biophys.Acta 1321, 207-220

Peter, H., Bader, A., Burkovski, A., Lambert, C., and Kramer, R. (1997)

Isolation of the putP gene of Corynebacterium glutamicum and characterization of a low-affinity uptake system for compatible solutes
Arch.Microbiol. 168, 143-151

Ruffert, S., Lambert, C., Peter, H., Wendisch, V. F., and Kramer, R. (1997)

Efflux of compatible solutes in Corynebacterium glutamicum mediated by osmoregulated channel activity
Eur.J.Biochem. 247, 572-580

Marienfeld, S., Uhlemann, E. M., Schmid, R., Kramer, R., and Burkovski, A. (1997)

Ultrastructure of the Corynebacterium glutamicum cell wall
Anton.Leeuwenhoek Ann.Microbiol. 72, 291-297

Jakoby, M., Tesch, M., Sahm, H., Kramer, R., and Burkovski, A. (1997)

Isolation of the Corynebacterium glutamicum glnA gene encoding glutamine synthetase I
FEMS Microbiol.Lett. 154, 81-88

Kramer, R. (1997)

The significance of transport reactions for amino acid production by bacteria
Proc.Int'l.Symp.Strain Development for Bioindustry 53-60

Kramer, R. (1997)

Bacterial transport systems: paradigms for the variety of options to catalyze solute transfer
Biol.Chem.H.-S. 378, S7

Burkovski, A. (1997)

Rapid detection of bacterial surface proteins using an enzyme-linked immunoadsorbent assay system
J.Biochem.Biophys.Methods 34, 69-71


Publications in 1996

Palmieri, L., Berns, D., Kramer, R., and Eikmanns, M. (1996)

Threonine diffusion and threonine transport in Corynebacterium glutamicum and their role in threonine production
Arch.Microbiol. 165, 48-54

Kramer, R. (1996)

Analysis and modeling of substrate uptake and product release by prokaryotic and eukaryotic cells
in: Metabolic Engineering (H.Sahm and C.Wandrey, eds.) vol. 54, p. 31-74, Springer, Heidelberg.

Kramer, R. (1996)

Genetic and physiological approaches for the production of amino acids
J.Biotechnol. 45, 1-21

Burkovski, A., Weil, B., and Kramer, R. (1996)

Characterization of a secondary uptake system for L-glutamate in Corynebacterium glutamicum
FEMS Lett. 136, 169-173

Kramer, R. (1996)

Carrier and channel properties of the mitochondrial phosphate translocator
Kidney Intern. 49, 947-952

Siewe, R., Weil, B., Burkovski, A., Eikmanns, B. J., Eikmanns, M., and Kramer, R. (1996)

Functional and genetic characterization of the (methyl)ammonium uptake carrier of Corynebacterium glutamicum
J.Biol.Chem. 271, 5398-5403

Kelle, R., Herrmann, T., Weuster-Botz, D., Eggeling, L., Kramer, R., and Wandrey, C. (1996)

Glucose-controlled L-isoleucine fed-batch production with recombinant strains of Corynebacterium glutamicum
J.Biotechnol. 50, 123-136

Kelle, R., Laufer, B., Brunzema, C., Weuster-Botz, D., Kramer, R., and Wandrey, C. (1996)

Reaction engineering analysis of L-lysine transport by Corynebacterium glutamicum
Biotechnol.Bioeng. 51, 40-50

Peekhaus, N. and Kramer, R. (1996)

The gluEMP operon from Zymomonas mobilis encodes a high-affinity glutamate carrier with similarity to binding-protein-dependent transport systems
Arch.Microbiol. 165, 325-332

Peter, H., Burkovski, A., and Kramer, R. (1996)

Isolation, characterization, and expression of the Corynebacterium glutamicum betP gene, encoding the transport system for the compatible solute glycine betaine
J.Bacteriol. 178, 5229-5234

Herrmann, T. and Kramer, R. (1996)

Mechanism and regulation of isoleucine excretion in Corynebacterium glutamicum
Appl.Environ.Microbiol. 62, 3238-3244

Sahm, H., Eggeling, L., Eikmanns, B., and Kramer, R. (1996)

Construction of L-lysine, L-threonine, or L-isoleucine overproducing strains of Corynebacterium glutamicum
in: Recombinant DNA Biotechnology III: The integration of biological and engineering sciences (J.A.Asenjo and B.A.Andrews, eds.) vol. 782, p. 25-39.

Eggeling, L., Kramer, R., Vrlijc, M., Kronemeyer, W., and Sahm, H. (1996)

Transport mutants and transport genes of Corynebacterium glutamicum
in: Recombinant DNA Biotechnology III: The integration of biological and engineering sciences (J.A.Asenjo and B.A.Andrews, eds.) vol. 782, p. 191-201.

Weisser, P., Kramer, R., and Sprenger, G. (1996)

Expression of the Escherichia coli pmi gene encoding phosphomannose-isomerase in Zymomonas mobilis leads to utilization of mannose as a novel growth substrate that can be used as selective marker
Appl.Environ.Microbiol. 62, 4155-4161

Wehrmann, A., Eggeling, L., Eikmanns, B., and Kramer, R. (1996)

Verfahren zur mikrobiellen Herstellung von Aminosaeuren mittels rekombinanter Mikroorganismen mit erhoehter Sekretionsrate
Patentanmeldung PCT / DE 96 / 01 162


Publications in 1995

Kronemeyer, W., Peekhaus, N., Kramer, R., Sahm, H., and Eggeling, L. (1995)

Structure of the gluABCD cluster encoding the glutamate uptake system of Corynebacterium glutamicum
J.Bacteriol. 177, 1152-1158

Herick, K., Stappen, R., and Kramer, R. (1995)

Comparison of functional and structural aspects of the reconstituted phosphate and aspartate/glutamate carrier from mitochondria
in: Thirty years of progress in mitochondrial bioenergetics and molecular biology (Palmieri, F., eds.) p. 83-87.

Burkovski, A. and Kramer, R. (1995)

Functional expression of the glutamate uptake system from Corynebacterium glutamicum in Escherichia coli
FEMS Microbiol.Lett. 127, 263-266

Sahm, H., Eggeling, L., Eikmanns, B., and Kramer, R. (1995)

Metabolic design in amino acid producing bacterium Corynebacterium glutamicum
FEMS Microbiol.Rev. 16, 243-252

Burkovski, A., Weil, B., and Kramer, R. (1995)

Glutamate excretion in Escherichia coli: dependency on the relA and spoT genotype
Arch.Microbiol. 164, 24-28

Weisser, P., Kramer, R., Sahm, H., and Sprenger, G. A. (1995)

Functional expression of the glucose transporter of Zymomonas mobilis leads to restoration of glucose and fructose uptake in Escherichia coli mutants and provides evidence for its facilitator action
J.Bacteriol. 177, 3351-3354

Herick, K. and Kramer, R. (1995)

Kinetic and energetic characterization of solute flux through the reconstituted aspartate/glutamate carrier from beef heart mitochondria after modification with mercurials
Biochim.Biophys.Acta 1238, 63-71

Siewe, R. M., Weil, B., and Kramer, R. (1995)

Glutamine uptake by a sodium-dependent secondary transport system in Corynebacterium glutamicum
Arch.Microbiol. 164, 98-103

Farwick, M., Siewe, R. M., and Kramer, R. (1995)

Glycine betaine uptake after hyperosmotic shift in Corynebacterium glutamicum
J.Bacteriol. 177, 4690-4695

Peekhaus, N., Tolner, B., Poolman, B., and Kramer, R. (1995)

The glutamate uptake regulatory protein (Grp) of Zymomonas mobilis and its relation to the global regulator Lrp of Escherichia coli
J.Bacteriol. 177, 5140-5147

Erdmann, A., Weil, B., and Kramer, R. (1995)

Regulation of lysine excretion in the lysine producer strain Corynebacterium glutamicum MH 20-22B
Biotechnol.Lett. 17, 927-932

Wehrmann, A., Morakkabati, S., Kramer, R., Sahm, H., and Eggeling, L. (1995)

Functional analysis of sequences adjacent to dapE of Corynebacterium glutamicum reveals the presence of aroP, which encodes the aromatic amino acid transporter
J.Bacteriol. 177, 5991-5993

Niederweis, M., Maier, E., Lichtinger, T., Benz, R., and Kramer, R. (1995)

Identification of channel-forming activity in the cell wall of Corynebacterium glutamicum
J.Bacteriol. 177, 5716-5718

Lambert, C., Erdmann, A., Eikmanns, M., and Kramer, R. (1995)

Triggering glutamate excretion in Corynebacterium glutamicum by modulating the membrane state by local anesthetics and osmotic gradients
Appl.Environ.Microbiol. 61, 4334-4342


Publications in 1994

Kramer, R. and Hoischen, C. (1994)

Futile cycling caused by the simultaneous presence of separate transport systems for uptake and secretion of amino acids in Corynebacterium glutamicum
in: Biothermokinetics (H.Westerhoff, eds.) p. 19-26, Intercept Publ.

Kramer, R. (1994)

Functional principles of solute transport systems - concepts and perspectives
Biochim.Biophys.Acta 1185, 1-34

Kramer, R., de Graaf, A., Eggeling, L., Eikmanns, B., and Sahm, H. (1994)

Biosynthesis and secretion of amino acids
in: Proc. 6th Eur. Congr. Biotechnol. (L.Alberghina, L.Frontali, and P.Sensi, eds.) p. 595-602, Elsevier, Amsterdam.

Kramer, R. (1994)

Secretion of amino acids by bacteria - physiology and mechanism
FEMS Microbiol.Rev. 13, 75-93

Stappen, R. and Kramer, R. (1994)

Kinetic mechanism of phosphate/phosphate and phosphate/OH- antiports catalyzed by reconstituted phosphate carrier from beef heart mitochondria
J.Biol.Chem.269 269, 11240-11246

Engel, P., Kramer, R., and Unden, G. (1994)

Transport of C-4-dicarboxylates by anaerobically grown Escherichia coli - Energetics and mechanism of exchange, uptake and efflux
Eur.J.Biochem. 222, 605-614

Kramer, R. (1994)

Systems and mechanisms of amino acid uptake and secretion in prokaryotes
Arch.Microbiol. 162, 1-13

Kramer, R., Boles, E., Eggeling, L., Erdmann, A., Gutmann, M., Kronemeyer, W., Palmieri, L., and Zittrich, S. (1994)

Mechanism and energetics of amino-acid transport in coryneform bacteria
Biochim.Biophys.Acta 1187, 245-249

Erdmann, A., Weil, B., and Kramer, R. (1994)

Lysine secretion by Corynebacterium glutamicum wild type: regulation of secretion carrier activity
Appl.Microbiol.Biotechnol. 42, 604-610

Broeer, S., Kronemeyer, W., Kramer, R., Eggeling, L., and Sahm, H. (1994)

Fermentationsverfahren zur Gewinnung von Aminosaeuren und dafuer geeigneter Bakterienstamm
Deutsches Patent DE 42.03.320 C2

Visser, W., Van der Baan, A. A., Batenburg-van der Egte, W., Scheffers, W. A., Kramer, R., and van Dijken, J. P. (1994)

Involvement of mitochondria in the assimilatory metabolism of anaerobic Saccharomyces cerevisiae cultures
Microbiology - UK 140, 3039-3046

Zittrich, S. and Kramer, R. (1994)

Quantitative discrimination of carrier-mediated excretion of isoleucine from uptake and diffusion in Corynebacterium glutamicum
J.Bacteriol. 176, 6892-6899

Ruhrmann, J., Sprenger, G. A., and Kramer, R. (1994)

Mechanism of alanine excretion in recombinant strains of Zymomonas mobilis
Biochim.Biophys.Acta 1196, 14-20

Loos, H., Kramer, R., Sahm, H., and Sprenger, G. A. (1994)

Sorbitol promotes growth of Zymomonas mobilis in environments with high concentrations of sugar: Evidence for a physiological function of glucose-fructose oxidoreductase in osmoprotection
J.Bacteriol. 176, 7688-7693